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        ImmunoChemistry:魔力紅組織蛋白酶B活性分析試劑盒

        發布者:艾美捷科技    發布時間:2022-11-29     
        分享到:

               組織蛋白酶B (Cathepsin B, CTSB)是一種廣泛表達于溶酶體上的半胱氨酸肽鏈內切酶,屬于木瓜蛋白酶家族的成員,廣泛存在于從病毒到哺乳動物和人等生物體內??赏ㄟ^參與細胞凋亡、細胞外基質降解、抗原呈遞、炎癥反應、細胞自噬等多種病理生理過程從而參與多種疾病的發生和發展。   


        組織蛋白酶B


               市面上常見的是檢測細胞裂解液中的組織蛋白酶B活性,更多研究者希望動態檢測 活細胞 內的組織蛋白酶B的活性,做更真實的實驗!艾美捷科技為您推薦Immunochemistry Tech(ICT)品牌專利Magic Red系列產品。 

               

               ICT的Magic Red?檢測法使研究人員能夠在體外對細胞內的蛋白酶活性進行定量和監測。


        貨號名稱規格樣本
        ICT-937Magic Red Cathepsin-B Assay Kit 魔力紅組織蛋白酶B活性分析試劑盒25T培養的活細胞 (懸浮和貼壁)
        ICT-938100T

        * 僅供科研使用,不得用于醫療. 

               

               【原理】Magic Red魔力紅底物,是細胞滲透性的,它很容易穿透細胞膜和內部細胞器的膜——不需要裂解或滲透步驟。如果組織蛋白酶是活性的,魔紅底物被裂解,甲酰紫熒光團將在激發時變為熒光(Ex/Em =592nm/628nm)。隨著酶活性的進展和更多魔紅底物的裂解,信號將增強,使研究人員能夠觀察顏色隨時間的變化。樣品可通過熒光顯微鏡熒光酶標儀進行分析。試劑盒中包括Hoechst 33342和吖啶橙,分別用于檢測核形態和溶酶體細胞器結構。   


               【產品組分】

        Magic Red Cathepsin-B Assay Kit 魔力紅組織蛋白酶B活性分析試劑盒
        25T100T
        l Magic Red Substrate (MR-RR2), 1 25 Test Vial, #6133 l Hoechst 33342, 1 mL, #639 l Acridine Orange, 0.5 mL, #6130 l Kit Manuall Magic Red Substrate (MR-RR2), 1 100 Test Vial, #6134 l Hoechst 33342, 1 mL, #639 l Acridine Orange, 0.5 mL, #6130 l Kit Manual
        保存條件:2-8°C 避光保存。
        點擊下載說明書PDF

         

                【Nature文章鑒賞】 

                IF=28.2, 《Lysosomal retargeting of Myoferlin mitigates membrane stress to enable pancreatic cancer growth》,Nature Cell Biology 


        魔力紅?組織蛋白酶B活性分析試劑盒

        魔力紅?組織蛋白酶B活性分析試劑盒


               Magic Red?相關產品列表:

        貨號品牌名稱規格(T)
        ICT-935ImmunochemistryMagic Red Caspase 3 & 7 Assay Kit25
        ICT-936ImmunochemistryMagic Red Caspase 3 & 7 Assay Kit100
        ICT-937ImmunochemistryMagic Red Cathepsin B Assay Kit25
        ICT-938ImmunochemistryMagic Red Cathepsin B Assay Kit100
        ICT-939ImmunochemistryMagic Red  Cathepsin K Assay Kit25
        ICT-940ImmunochemistryMagic Red  Cathepsin K Assay Kit100
        ICT-941ImmunochemistryMagic Red Cathepsin L Assay Kit25
        ICT-942ImmunochemistryMagic Red Cathepsin L Assay Kit100

         

               【Magic Red Cathepsin-B Assay Kit,更多發表文章,28+】 

               1、Hao, W;Dian, M;Zhou, Y;Zhong, Q;Pang, W;Li, Z;Zhao, Y;Ma, J;Lin, X;Luo, R;Li, Y;Jia, J;Shen, H;Huang, S;Dai, G;Wang, J;Sun, Y;Xiao, D. Autophagy induction promoted by m6A reader YTHDF3 through translation upregulation of FOXO3 mRNA. Nature communications. 2022 October 4; doi: 10.1038/s41467-022-32963-0. Article   

               2、Li, Y;Xu, B;Ren, X;Wang, L;Xu, Y;Zhao, Y;Yang, C;Yuan, C;Li, H;Tong, X;Wang, Y;Du, J. Inhibition of CISD2 promotes ferroptosis through ferritinophagy-mediated ferritin turnover and regulation of p62-Keap1-NRF2 pathway. Cellular & molecular biology letters. 2022 September 30; doi: 10.1186/s11658-022-00383-z. Full Abstract   

               3、Iyer, H;Shen, K;Meireles, AM;Talbot, WS. A lysosomal regulatory circuit essential for the development and function of microglia. Science Advances. 2022 September 2; doi: 10.1126/sciadv.abp8321. Abstract  

               4、Akter, E;Tasaki, Y;Mori, Y;Nakai, K;Hachiya, K;Lin, H;Konno, M;Kamasaki, T;Tanabe, K;Umeda, Y;Yamano, S;Fujita, Y;Kon, S. Non-degradable autophagic vacuoles are indispensable for cell competition. Cell reports. 2022 August 30; doi: 10.1016/j.celrep.2022.111292. Article   

               5、Fernandez, MA;Bah, F;Ma, L;Lee, Y;Schmidt, M;Welch, E;Morrow, EM;Young-Pearse, TL. Loss of endosomal exchanger NHE6 leads to pathological changes in tau in human neurons. Stem cell reports. 2022 August 17; doi: 10.1016/j.stemcr.2022.08.001. Full Report   

               6、Ratto, E;Chowdhury, SR;Siefert, NS;Schneider, M;Wittmann, M;Helm, D;Palm, W. Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly. Nature communications. 2022 August 17; doi: 10.1038/s41467-022-32515-6. Article   

               7、Oe, Y;Kakuda, K;Yoshimura, SI;Hara, N;Hasegawa, J;Terawaki, S;Kimura, Y;Ikenaka, K;Suetsugu, S;Mochizuki, H;Yoshimori, T;Nakamura, S. PACSIN1 is indispensable for amphisome-lysosome fusion during basal autophagy and subsets of selective autophagy. PLoS Genetics. 2022 Jun 1; doi: 10.1371/journal.pgen.1010264. Text  

               8、Hu, M;Li, P;Wang, C;Feng, X;Geng, Q;Chen, W;Marthi, M;Zhang, W;Gao, C;Reid, W;Swanson, J;Du, W;Hume, RI;Xu, H. Parkinson's disease-risk protein TMEM175 is a proton-activated proton channel in lysosomes. Cell. 2022 June 23; doi: 10.1016/j.cell.2022.05.021. Full Text   

               9、Tanno, T;Zhang, P;Bailey, C;Wang, Y;Ittiprasert, W;Devenport, M;Zheng, P;Liu, Y. A novel aptamer-based small RNA delivery platform and its application to cancer therapy. Genes & Diseases. 2022 May 1; doi: 10.1016/j.gendis.2022.05.004. Full Text   

               10、Zhong, W;Rao, Z;Xu, J;Sun, Y;Hu, H;Wang, P;Xia, Y;Pan, X;Tang, W;Chen, Z;Zhou, H;Wang, X. Defective mitophagy in aged macrophages promotes mitochondrial DNA cytosolic leakage to activate STING signaling during liver sterile inflammation. Aging Cell. 2022 May 22; doi: 10.1111/jcpe.13647. Abstract   

               11、Browning, CL;Green, A;Gray, EP;Hurt, R;Kane, AB. Manganese dioxide nanosheets induce mitochondrial toxicity in fish gill epithelial cells. Nanotoxicology. 2021 Jan 27; 1-18. doi: 10.1080/17435390.2021.1874562. Article   

               12、Lan J, Luo H, Wu R, Wang J, Zhou B, Zhang Y, Jiang Y, Xu J. Internalization of HMGB1 (High Mobility Group Box 1) Promotes Angiogenesis in Endothelial Cells. Arterioscler Thromb Vasc Biol. 2020 Dec;40(12):2922-2940. doi: 10.1161/ATVBAHA.120.315151. Epub 2020 Oct 1. Full Text  

               13、J?ger E, Murthy S, Schmidt C, Hahn M, Strobel S, Peters A, St?ubert C, Sungur P, Venus T, Geisler M, Radusheva V, Raps S, Rothe K, Scholz R, Jung S, Wagner S, Pierer M, Seifert O, Chang W, Estrela-Lopis I, Raulien N, Krohn K, Str?ter N, Hoeppener S, Sch?neberg T, Rossol M, Wagner U. Calcium-sensing receptor-mediated NLRP3 inflammasome response to calciprotein particles drives inflammation in rheumatoid arthritis. Nat Commun. 2020 Aug 25;11(1):4243. doi: 10.1038/s41467-020-17749-6. Full Text  

               14、Yin Q, Jian Y, Xu M, Huang X, Wang N, Liu Z, Li Q, Li J, Zhou H, Xu L, Wang Y, Yang C. CDK4/6 regulate lysosome biogenesis through TFEB/TFE3. J Cell Biol. 2020 Aug 3;219(8):e201911036. doi: 10.1083/jcb.201911036. Full Text   

               15、Almacellas E, Pelletier J, Day C, Ambrosio S, Tauler A, Mauvezin C. Lysosomal degradation ensures accurate chromosomal segregation to prevent chromosomal instability. Autophagy. 2020 Jun 23;1-18. doi: 10.1080/15548627.2020.1764727. Online ahead of print. Full Text   

               16、Song SB, Hwang ES. High Levels of ROS Impair Lysosomal Acidity and Autophagy Flux in Glucose-Deprived Fibroblasts by Activating ATM and Erk Pathways. Biomolecules. 2020 May 13;10(5):761. doi: 10.3390/biom10050761. Abstract   

               17、He M, Zhang T, Zhu Z, Qin S, Wang H, Zhao L, Zhang X, Hu J, Wen J, Cai H, Xin Q, Guo Q, Lin L, Zhou B, Zhang H, Xia G, Wang C. LSD1 contributes to programmed oocyte death by regulating the transcription of autophagy adaptor SQSTM1/p62. Aging Cell. 2020 Mar;19(3):e13102. doi: 10.1111/acel.13102. Epub 2020 Feb 19. Full Text   

               18、Cannata Serio M, Graham LA, Ashikov A, Larsen LE, Raymond K, Timal S, Le Meur G, Ryan M, Czarnowska E, Jansen JC, He M, Ficicioglu C, Pichurin P, Hasadsri L, Ríos-Ocampo WA, Gilissen C, Rodenburg R, Jonker JW, Holleboom AG, Morava E, Veltman JA, Socha P, Stevens TH, Simons M, Lefeber DJ. sMutations in the V-ATPase assembly factor VMA21 cause a congenital disorder of glycosylation with autophagic liver disease. Hepatology. 2020 Mar 7. doi: 10.1002/hep.31218. [Epub ahead of print]. Abstract   

               19、Kratschmer C, Levy M. Targeted Delivery of Auristatin Modified Toxins to Pancreatic Cancer Using Aptamers. Molecular Therapy-Nucleic Acids. 2017. 10:227-236 March 2018. doi.org/10.1016/j.omtn.2017.11.013. Full text   

               20、Hover S, Foster B, Fontana J, Kohl A, Goldstein SAN, Barr JN, Mankouri J. Bunyavirus requirement for endosomal K+ reveals new roles of cellular ion channels during infection. PLoS Pathog. 2018. Jan 19;14(1):e1006845. doi: 10.1371/journal.ppat.1006845. eCollection 2018 Jan. Full text   

               21、Festa BP, Chen Z, Berquez M, Debaix H, Tokonami N, Prange JA, Hoek GV, Alessio C, Raimondi A, Nevo N, Giles RH, Devuyst O, Luciani A. Impaired autophagy bridges lysosomal storage disease and epithelial dysfunction in the kidney. Nat Commun. 2018. Jan 11;9(1):161. doi: 10.1038/s41467-017-02536-7. Full text   

               22、Qian Q, Zhang Z, Orwig, A, Chen S, Ding WX, Xu Y, Kunz RC, Lind NRL, Stamler JS, Yang L. S-nitrosoglutathione Reductase Dysfunction Contributes to Obesity-Associated Hepatic Insulin Resistance via Regulating Autophagy. Diabetes. 2017. Oct 26. pii: db170223. doi: 10.2337/db17-0223. [Epub ahead of print]. Abstract  

               23、Chen J, Ou Y, Li Y, Hu S, Shao LW, Liu Y. Metformin extends C. elegans lifespan through lysosomal pathway. Elife. 2017.Oct 13;6. pii: e31268. doi: 10.7554/eLife.31268. Full Text  

               24、Ríos-Luci C, García-Alonso S, Díaz-Rodríguez E, Nadal-Serrano M, Arribas J, Oca?a A, Pandiella A. Resistance to the antibody-drug conjugate T-DM1 is based in a reduction in lysosomal proteolytic activity. Cancer Res. 2017 Jul 7. doi: 10.1158/0008-5472.CAN-16-3127. [Epub ahead of print]. Abstract.   

               25、Kang HT, Park JT, Choi K, Kim Y, Choi HJC, Jung CW, Lee YS, Chul Park S. Chemical screening identifies ATM as a target for alleviating senescence. Nat Chem Biol. 2017. Jun;13(6):616-623. Epub 2017 Mar 27. Abstract.   

               26、Sakamaki JI, Wilkinson S, Hahn M, Tasdemir N, O’Prey J, Clark W, Hedley A, Nixon C, Long JS, New M, Van Acker T, Tooze SA, Lowe SW, Dikic I, and Ryan KM. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol. Cell. 2017. May 18;66(4):517-532.e9. doi: 10.1016/j.molcel.2017.04.027. Full text.   

               27、Hamanaka T, Nishizawa K, Sakasegawa Y, Oguma A, Teruya K, Kurahashi H, Hara H, Sakaguchi S, Doh-Ura K. Melanin or melanin-like substance interacts with the N-terminal portion of prion protein and inhibits abnormal prion protein formation in prion-infected cells. J Virol. 2017. Abstract.  

               28、Fujita N, Huang W, Lin TH, Groulx JF, Jean S, Nguyen J, Kuchitsu Y, Koyama-Honda I, Mizushima N, Fukuda M, Kiger AA. Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. Elife. 2017. Full text.   


               美國ICT除了可提供全面的細胞活性檢測相關試劑,包括凋亡、 caspase、 自噬、細胞毒性、壞死等相關檢測,還可以同時提供高質量高靈敏度的 ELISA以及相關試劑,包括包被液、封閉液、 沖洗液、 底物、 終止液、 板子等等。    

               看到這兒,您心動了嗎?馬上聯系小艾吧! 

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